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Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus

Ruzek, D., Gritsun, T. S., Forrester, N. L., Gould, E. A., Kopecky, J., Golovchenko, M., Rudenko, N. and Grubhoffer, L. (2008) Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus. Virology, 374 (2). pp. 249-255. ISSN 0042-6822

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To link to this item DOI: 10.1016/j.virol.2008.01.010

Abstract/Summary

An attenuated strain (263) of the tick-borne encephalitis virus, isolated from field ticks, was either serially subcultured, 5 times in mice, or at 40 degrees C in PS cells, producing 2 independent strains, 263-m5 and 263-TR with identical genomes; both strains exhibited increased plaque size, neuroinvasiveness and temperature-resistance. Sequencing revealed two unique amino acid substitutions, one mapping close to the catalytic site of the viral protease. These observations imply that virus adaptation from ticks to mammals occurs by selection of pre-existing virulent variants from the quasispecies population rather than by the emergence of new random mutations. The significance of these observations is discussed. (c) 2008 Elsevier Inc. All rights reserved.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences
ID Code:9882
Uncontrolled Keywords:tick-borne encephalitis virus, neuroinvasiveness, viral protease, IXODES-RICINUS TICKS, ENVELOPE PROTEIN, VIRULENCE, TRANSMISSION, FLAVIVIRUS

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