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Genotypic and phenotypic diversity of a baculovirus population within an individual insect host

Cory, J. S., Green, B. M., Paul, R. K. and Hunter-Fujita, F. (2005) Genotypic and phenotypic diversity of a baculovirus population within an individual insect host. Journal of Invertebrate Pathology, 89 (2). pp. 101-111. ISSN 0022-2011

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To link to this item DOI: 10.1016/j.jip.2005.03.008


It is becoming increasingly apparent that many pathogen populations, including those of insects, show high levels of genotypic variation. Baculoviruses are known to be highly variable, with isolates collected from the same species in different geographical locations frequently showing genetic variation and differences in their biology. More recent Studies at smaller scales have also shown that virus DNA profiles from individual larvae can show polymorphisms within and between populations of the same species. Here, we investigate the genotypic and phenotypic variation of an insect baculovirus infection within a single insect host. Twenty four genotypically distinct nucleopolyhedrovirus (NPV) variants were isolated from an individual pine beauty moth, Panolis flammea, caterpillar by in vivo cloning techniques. No variant appeared to be dominant in the population. The Pafl NPV variants have been mapped using three restriction endonucleases and shown to contain three hypervariable regions containing insertions of 70-750 bp. Comparison of seven of these variants in an alternative host, Mamestra brassicae, demonstrated that the variants differed significantly in both pathogenicity and speed of kill. The generation and maintenance of pathogen heterogeneity are discussed. (c) 2005 Elsevier Inc. All rights reserved.

Item Type:Article
Divisions:Life Sciences > School of Biological Sciences
ID Code:10296
Uncontrolled Keywords:baculovirus, diversity, genotype, hypervariable regions, pathogenicity, polymorphism, population structure, quasispecies, speed of kill, variation, virulence, NUCLEAR POLYHEDROSIS-VIRUS, RESTRICTION-ENDONUCLEASE ANALYSIS, CYDIA-POMONELLA GRANULOVIRUS, PINE BEAUTY MOTH, PANOLIS-FLAMMEA, GRANULOSIS-VIRUS, HOMOLOGOUS RECOMBINATION, GENETIC-HETEROGENEITY, TRANSPOSON TCP3.2, GENOME SEQUENCE

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