Identification of lipophilic flavones and flavonols by comparative HPLC, TLC and UV spectral analysis

Greenham, J., Harborne, J.B. and Williams, C.A. (2003) Identification of lipophilic flavones and flavonols by comparative HPLC, TLC and UV spectral analysis. Phytochemical Analysis, 14 (2). pp. 100-118. ISSN 0958-0344 doi: 10.1002/pca.693

Abstract/Summary

The identification of lipophilic flavones and flavonols using a combination of high performance liquid chromatography, thin layer chromatography and UV spectral analysis is discussed. Data are provided for the flavones, apigenin, luteolin and tricetin and twelve of their methyl ethers, 8-hydroxyluteolin, 6-hydroxyluteolin and scutellarein and fourteen of their methyl ethers, and some 6,8-dihydroxyapigenin and 6,8-dihydroxyluteolin derivatives. Data for some forty two flavonols with extra 6- and/or 8-hydroxylation, mostly 6-hydroxykaempferol and quercetagetin derivatives, are also presented. The remaining compounds analysed include fourteen 5-deoxyflavones, four 5-methoxyflavones and five 5-deoxyflavonols plus further 5-hydroxylated flavones and flavonols without B-ring oxidation or with 2-, 5- or 6-hydroxylation. Copyright © 2003 John Wiley & Sons, Ltd.