The iron–sulfur cluster of Bacterioferritin‐associated ferredoxin (Bfd): a “biological fuse” that prevents oxidative damage to cells?

Bradley, J. M., Carter, A. M., Bugg, Z., Andrews, S. C.E. ORCID: https://orcid.org/0000-0003-4295-2686 and Le Brun, N. E. ORCID: https://orcid.org/0000-0001-9780-4061 (2025) The iron–sulfur cluster of Bacterioferritin‐associated ferredoxin (Bfd): a “biological fuse” that prevents oxidative damage to cells? Angewandte Chemie International Edition, 64 (34). e202511340. ISSN 1433-7851 doi: 10.1002/anie.202511340

Abstract/Summary

Iron is in an essential micronutrient in living systems. However, it is also potentially toxic and so the concentration of chelatable iron within cells is tightly regulated to prevent catalytic formation of harmful reactive oxygen species (ROS). Ferritins play a key role in iron homeostasis by storing excess iron as an insoluble ferric mineral within the protein. When bacterial cells become iron deficient, this store may be accessed by reduction/solubilisation of the iron. Bacterioferritins utilise heme, bound at an inter‐subunit site, to support electron transfer to the stored mineral. Electrons for heme reduction are shuttled from NADPH via Bfd, a [2Fe–2S] cluster‐containing ferredoxin. This raises the paradox that the synthesis of an iron‐dependent protein co‐factor is required under conditions of iron‐deficiency so that stored iron can be utilised. Here, we show that exposure of Bfd to ROS suppresses the capacity of the protein to stimulate iron release from bacterioferritin. We propose that reliance of iron release on Bfd evolved to ensure that chelatable iron levels do not increase under oxidative stress conditions. Thus, the Bfd iron–sulfur cluster functions as a “biological fuse” in providing a fail‐safe that immediately halts iron release once ROS accumulate to damaging concentrations.

Item Type	Article
URI	https://centaur.reading.ac.uk/id/eprint/123454
Identification Number/DOI	10.1002/anie.202511340
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	Wiley
Download/View statistics	View download statistics for this item