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Expression and function of the bile acid receptor GpBAR1 (TGR5) in the murine enteric nervous system

Poole, D.P., Godfrey, C., Cattaruzza, F., Cottrell, G.S., Kirkland, J.G., Pelayo, J.C., Bunnett, N.W. and Corvera, C.U. (2010) Expression and function of the bile acid receptor GpBAR1 (TGR5) in the murine enteric nervous system. Neurogastroenterology and Motility, 22 (7). 814-e228. ISSN 1365-2982

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To link to this item DOI: 10.1111/j.1365-2982.2010.01487.x


BACKGROUND: Bile acids (BAs) regulate cells by activating nuclear and membrane-bound receptors. G protein coupled bile acid receptor 1 (GpBAR1) is a membrane-bound G-protein-coupled receptor that can mediate the rapid, transcription-independent actions of BAs. Although BAs have well-known actions on motility and secretion, nothing is known about the localization and function of GpBAR1 in the gastrointestinal tract. METHODS: We generated an antibody to the C-terminus of human GpBAR1, and characterized the antibody by immunofluorescence and Western blotting of HEK293-GpBAR1-GFP cells. We localized GpBAR1 immunoreactivity (IR) and mRNA in the mouse intestine, and determined the mechanism by which BAs activate GpBAR1 to regulate intestinal motility. KEY RESULTS: The GpBAR1 antibody specifically detected GpBAR1-GFP at the plasma membrane of HEK293 cells, and interacted with proteins corresponding in mass to the GpBAR1-GFP fusion protein. GpBAR1-IR and mRNA were detected in enteric ganglia of the mouse stomach and small and large intestine, and in the muscularis externa and mucosa of the small intestine. Within the myenteric plexus of the intestine, GpBAR1-IR was localized to approximately 50% of all neurons and to >80% of inhibitory motor neurons and descending interneurons expressing nitric oxide synthase. Deoxycholic acid, a GpBAR1 agonist, caused a rapid and sustained inhibition of spontaneous phasic activity of isolated segments of ileum and colon by a neurogenic, cholinergic and nitrergic mechanism, and delayed gastrointestinal transit. CONCLUSIONS & INFERENCES: G protein coupled bile acid receptor 1 is unexpectedly expressed in enteric neurons. Bile acids activate GpBAR1 on inhibitory motor neurons to release nitric oxide and suppress motility, revealing a novel mechanism for the actions of BAs on intestinal motility.

Item Type:Article
Divisions:No Reading authors. Back catalogue items
ID Code:30253
Uncontrolled Keywords:Animals Blotting, Western Cell Line Cyclic AMP/biosynthesis Enteric Nervous System/*metabolism Fluorescent Antibody Technique Gastric Emptying Gastrointestinal Motility Gastrointestinal Tract/anatomy & histology/metabolism Immunohistochemistry Intestines/innervation Male Mice Mice, Inbred C57BL Microscopy, Confocal Motor Neurons/physiology Myenteric Plexus/metabolism Nitric Oxide/physiology RNA/biosynthesis/genetics Receptors, G-Protein-Coupled/*biosynthesis/*genetics Reverse Transcriptase Polymerase Chain Reaction
Additional Information:Poole, D P Godfrey, C Cattaruzza, F Cottrell, G S Kirkland, J G Pelayo, J C Bunnett, N W Corvera, C U DK026743/DK/NIDDK NIH HHS/ DK07573/DK/NIDDK NIH HHS/ DK39957/DK/NIDDK NIH HHS/ DK43207/DK/NIDDK NIH HHS/ DK57840/DK/NIDDK NIH HHS/ P30 DK026743-26A1/DK/NIDDK NIH HHS/ P30 DK026743-27/DK/NIDDK NIH HHS/ England Neurogastroenterol Motil. 2010 Jul;22(7):814-25, e227-8. Epub 2010 Mar 12.


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