Accessibility navigation

Mammalian skeletal muscle fibres promote non-muscle stem cells and non-stem cells to adopt myogenic characteristics

Morash, T., Henry, C.-H., Mitchell, R. and Patel, K. (2017) Mammalian skeletal muscle fibres promote non-muscle stem cells and non-stem cells to adopt myogenic characteristics. Fibers, 5 (1). 5. ISSN 2079-6439

Text (Open Access) - Published Version
· Available under License Creative Commons Attribution.
· Please see our End User Agreement before downloading.

[img] Text - Accepted Version
· Restricted to Repository staff only


It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.3390/fib5010005


Skeletal muscle fibres are unique cells in large animals, often composed of thousands of post-mitotic nuclei. Following skeletal muscle damage, resident stem cells called satellite cells commit to myogenic differentiation and migrate to carry out repair. Satellite stem cells migrate on muscle fibres through amoeboid movement which relies on dynamic cell membrane extension and retraction (blebbing). It is not known whether blebbing is due to intrinsic properties of satellite cells or induced by features of the myofibre surface. Here we determined the influence of the muscle fibre matrix on two important features of muscle regeneration: the ability to migrate and to differentiate down a myogenic lineage. We show that the muscle fibre is able to induce amoeboid movement in non-muscle stem cells and non-stem cells. Secondly we show that prolonged co-culture on myofibres caused amniotic fluid stem cells and breast cancer cells to express MyoD, a key myogenic determinant. Finally we show that amniotic fluid stem cells co-cultured on myofibres are able to fuse and make myotubes that express Myosin Heavy Chain.

Item Type:Article
Divisions:Life Sciences > School of Biological Sciences > Biomedical Sciences
ID Code:68682


Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation