Selection of candidate coding DNA barcoding regions for use on land plantsFord, C. S., Ayres, K. L., Toomey, N., Haider, N., Van Alphen Stahl, J., Kelly, L. J., Wikström, N., Hollingsworth, P. M., Duff, R. J., Hoot, S. B., Cowan, R. S., Chase, M. W. and Wilkinson, M. J. (2009) Selection of candidate coding DNA barcoding regions for use on land plants. Botanical Journal of the Linnean Society, 159 (1). pp. 1-11. ISSN 0024-4074 Full text not archived in this repository. It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1111/j.1095-8339.2008.00938.x Abstract/SummaryAn in silico screen of 41 of the 81 coding regions of the Nicotiana plastid genome generated a shortlist of 12 candidates as DNA barcoding loci for land plants. These loci were evaluated for amplification and sequence variation against a reference set of 98 land plant taxa. The deployment of multiple primers and a modified multiplexed tandem polymerase chain reaction yielded 85–94% amplification across taxa, and mean sequence differences between sister taxa of 6.1 from 156 bases of accD to 22 from 493 bases of matK. We conclude that loci should be combined for effective diagnosis, and recommend further investigation of the following six loci: matK, rpoB, rpoC1, ndhJ, ycf5 and accD.
Altmetric Deposit Details University Staff: Request a correction | Centaur Editors: Update this record |
Lists
Lists
