A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells
Hall, R. D., Riksen-Bruinsma, T., Weyens, G. J., Rosquin, I. J., Denys, P. N., Evans, I. J., Lathouwers, J. E., Lefèbvre, M. P., Dunwell, J. M. Full text not archived in this repository. It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1038/nbt0996-1133 Abstract/SummaryAn optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L.). A polyethylene glycol-mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.
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