Effect of anther orientation on microspore-callus production in barley (Hordeum vulgate L.)
Shannon, P. R. M., Nicholson, A. E., Dunwell, J. M. Full text not archived in this repository. It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1007/BF00040201 Abstract/SummaryBarley anthers from cold pretreated spikes produced no or few calluses when lated with both loculi in contact with the medium ('flat'). When anthers were plated with only one loculus in contact with the medium ('up'), a high proportion of the anthers produced calluses. The top loculus of the 'up' anthers was most productive. 'Flat' anthers, when compared with 'up' anthers, were not only slower to produce multicellular pollen grains (MCPs) and microcalluses, but also produced fewer of them and ceased production earlier. The MCPs and microcalluses in 'flat' anthers grew more slowly and few developed beyond the 30 cell stage. These results establish the importance of anther orientation for barley anther culture.
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