In vitro regeneration from seedling organs of Brassica oleracea var. italica Plenck cv. Green Comet II. Effect of light conditions and explant size

Lists

Tools

Lazzeri, P. A. and Dunwell, J. M. ORCID: https://orcid.org/0000-0003-2147-665X (1986) In vitro regeneration from seedling organs of Brassica oleracea var. italica Plenck cv. Green Comet II. Effect of light conditions and explant size. Annals of Botany, 58 (5). pp. 699-710. ISSN 1095-8290

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

Official URL: https://academic.oup.com/aob/article/58/5/699/1424...

Abstract/Summary

The calabrese cultivar Brassica oleracea var. italica cv. Green Comet was used in a study of the effects of various physical and environmental factors on growth and differentiation of seedling organs in vitro. The particular factors studied were explant size, light conditions during seedling growth and during explant incubation and high and low temperature preculture treatments. For all explants, frequencies of survival and differentiation increased with increase in explant size (e.g. 7 mm discs produced ten times more shoots per disc than 4 mm discs). Although seedling morphology was strongly affected by the different light regimes there was no consistent effect on the subsequent response of seedling explants in vitro. Shoot formation from leaf discs and hypocotyl segments was better at 30 μE m-1 s-1 than at 100 or 200 μE m-1 s-1 (white fluorescent tubes), but more shoots were formed on root segments at the high than at low light intensities. For all three types of explants, shoot production was better with Grolux light at 32 μE m-1 s-1 than with white light at 30 μE m-1 s-1. Experiments involving preculture at high (35 °C or low (4 °C temperature showed no consistent effects.

Item Type:	Article
Refereed:	Yes
Divisions:	Life Sciences > School of Agriculture, Policy and Development > Department of Crop Science
ID Code:	86977
Uncontrolled Keywords:	Brassica oleracea var. italica, calabrese, tissue culture, seedling, shoot regeneration
Publisher:	Oxford University Press

Deposit Details

References

CASSELS, A.C, LONG, R.D. and MOUSDALE, D.M.A., 1982. Endogenous LAA and morphogenesis in tobacco petiole cultures. Physiologia Plantarum 56, 507-12. DUNWELL, J.M., 1981. In vitro regeneration from excised leaf discs of three Brassica species. Journal of Experimental Botany 32, 789-99. FONNESBECH, M., 1974. Temperature effects on shoot and root development from Begonia x cheimantha petiole segments grown in vitro. Physiologia Plantarum 32, 282-6. GEORGE, L. and RAO, P.S., 1982. In vitro induction of pollen embryos and plantlets in Brassica juncea through anther culture. Plant Science Letters 26, 111-6. HAMMERSCHLAG, F. A., 1978. Influence of light intensity and date of explantation on growth of Geranium callus. Journal of Horticultural Science 13, 153-4. HANSEN, J. and ERNSTEN, A., 1982. Seasonal change in adventitious root formation in hypocotyl cuttings of Pinus sylvestris: influence of photoperiod during stock plant growth and of IBA treatment of cuttings. Physiologia Plantarum 54, 99-106. HASEGAWA, P.M., MURASHIGB, T., and TAKTORI, F. H., 1973. Propagation of Asparagus through shoot apex culture. II. Light and temperature requirements, transplantability of plants and cytological characteristics. Journal of The American Society of Horticultural Science 98, 143-8. HELSON, V.A., 1965. Comparison of Grolux and cool white fluorescent lamps with and without incandescence as light sources used in growth rooms for growth and development of tomato plants. Canadian Journal of Plant Science 45, 461-6. HUGHES, K.W., 1981. In vitro ecology - exogenous factors affecting growth and morphogenesis in plant culture systems. Environmental and Experimental Botany 21, 281-8. HUXTER, T.J., THORPE, T.A. and REID, D.M., 1981. Shoot initiation in light- and dark-grown tobacco callus: the role of ethylene. Physiologia Plantarum 53, 319-26. INAGAKI, N., HARADA, T., and YAKUWA, T., 1982. Studies on the anther culture of horticultural crops. VI. Regeneration of plantlets from shoots obtained through the anther culture of Asparagus officinalis L. Journal of the Faculty of Agriculture, University of Hokkaido 60, 275—83. KATO, Y., 1978. The involvement of photosynthesis in inducing bud formation on excised leaf segments of Heloniopsis orientals (Liliaceae). Plant and Cell Physiology 19, 791-9. KATO, Y., and OZAWA, N., 1979. Adventitious bud formation on leaf and stem segments of Heloniopsis orientalis grown at various temperatures. Plant and Cell Physiology 20, 491-7. KELLER, W.A. and ARMSTRONG, K.C , 1983. Production of haploids via anther culture in Brassica oleracea var. italica. Euphytica 32, 151-9. LAZZERI, P.A. and DUNWELL, J.M., 1986. In vitro regeneration from seedling tissues of Brassica oleracea var. italica Plenck cv. Green Comet. I. Effect of exogenous plant growth regulators. Annals of Botany 58, 689-697. MARGARA, J., 1969. Etude des facteurs de la neoformation de bourgeons en culture in vitro chez le chou-fleur Brassica oleracea L. var botrytis. Annales des Physiologie Vegetale 11, 95-112. MURASHIGE, T., 1977. Manipulation of organ initiation in plant tissue cultures. Botanical Bulletin of Academia Sinica 18, 1-24. MURASHIGE, T., and SKOOG, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473-97. NIMI, Y., 1978. Influence of low and high temperatures on the initiation and the development of bulb primordia in isolated tulip embryos. Scientia Horticulturae 9, 61-9. PETERSON, K.E. and ROST, T.L., 1979. Effects of light and hormones on regeneration of Crassula argentea from leaf discs. American Journal of Botany 66, 463-70. PHILIPS, G.C , and COLLINS, G.B., 1979. Virus symptom-free plants from clover using meristem culture. Crop Science 19,213-16. SCHLEGEL, G. and SCHNEIDER-MAESSEN, R., 1981. Einfluss von Licht auf die Regeneration von Saintpaulia ionantha H. Wendl in vitro. Gartenbauwissenschaft 46, 106-14. TRAN THAN VAN, K., 1980. Thin cell layers - control of morphogenesis by inherent factors and exogenously applied factors. International Review of Cytology, Supplement 11 A, 175-94. YEOMAN, M.M. and DAVIDSON, A.W., 1971. Effect of light on cell division in developing callus cultures. Annals of Botany 35, 1085-100.

University Staff: Request a correction | Centaur Editors: Update this record

University of Reading

CentAUR: Central Archive at the University of Reading

Accessibility navigation

In vitro regeneration from seedling organs of Brassica oleracea var. italica Plenck cv. Green Comet II. Effect of light conditions and explant size

Abstract/Summary

Page navigation

See also

Footer navigation