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Anther culture with different genotypes of opium poppy (Papaver somniferum L.): effect of cold treatment

Dieu, P. and Dunwell, J. M. (1988) Anther culture with different genotypes of opium poppy (Papaver somniferum L.): effect of cold treatment. Plant Cell, Tissue and Organ Culture, 12 (3). pp. 263-271. ISSN 0167-6857

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To link to this item DOI: 10.1007/BF00034367


This study concerns anther culture and the production of microspore-derived calluses and plants of the opium poppy (Papaver somniferum L.). It was confirmed that growth regulators were necessary for microspore callus production. Cold treatment (7 d at 7 °C) of the buds prior to culture lead to a twofold increase in the frequency of responsive anthers and in the number of calluses per 100 anthers plated. Callus was produced from cultured anthers of several genotypes, covering a wide genetic background. Step by step removal of growth regulators from the culture medium promoted organogenesis and plant regeneration. Most regenerated plants were diploid. The overall process of microspore embryogenesis closely resembled that described in previous reports on somatic callus production and plant regeneration from poppy hypocotyls in vitro.

Item Type:Article
Divisions:Life Sciences > School of Agriculture, Policy and Development > Biodiversity, Crops and Agroecosystems Division > Crops Research Group
ID Code:86984
Uncontrolled Keywords:Papaver somniferum, poppy, anther, pollen, haploid.

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