Rapid acidification and alkylation: Redox analysis of the MHC class I pathwayPowis, S. J., Nesbeth, D., Lenart, I., Fussell, H., Lamb, T., Gould, K. and Antoniou, A. N. (2009) Rapid acidification and alkylation: Redox analysis of the MHC class I pathway. Journal of Immunological Methods, 340 (1). pp. 81-85. ISSN 0022-1759 Full text not archived in this repository. It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1016/j.jim.2008.09.006 Abstract/SummaryThe technique of rapid acidification and alkylation can be used to characterise the redox status of oxidoreductases, and to determine numbers of free cysteine residues within substrate proteins. We have previously used this method to analyse interacting components of the MHC class I pathway, namely ERp57 and tapasin. Here, we have applied rapid acidification alkylation as a novel approach to analysing the redox status of MHC class I molecules. This analysis of the redox status of the MHC class I molecules HLA-A2 and HLA-B27, which is strongly associated with a group of inflammatory arthritic disorders referred to as Spondyloarthropathies, revealed structural and conformational information. We propose that this assay provides a useful tool in the study of in vivo MHC class I structure. (c) 2008 Elsevier B.V. All rights reserved.
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