Accessibility navigation

Sex estimation of teeth at different developmental stages using dimorphic enamel peptide analysis

Gowland, R., Stewart, N. A., Crowder, K. D., Hodson, C. ORCID:, Shaw, H., Gron, K. J. and Montgomery, J. (2021) Sex estimation of teeth at different developmental stages using dimorphic enamel peptide analysis. American Journal of Physical Anthropology, 174. pp. 859-869. ISSN 0002-9483

Text (Open Access) - Published Version
· Available under License Creative Commons Attribution.
· Please see our End User Agreement before downloading.


It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1002/ajpa.24231


Objectives: This study tests, for the first time, the applicability of a new method of sex estimation utilizing enamel peptides on a sample of deciduous and permanent teeth at different stages of mineralization, from nonadults of unknown sex, including perinates. Materials and methods: A total of 43 teeth from 29 nonadult individuals aged from 40 gestational weeks to 19 years old were analyzed. The sample included pairs of fully mineralized and just developing teeth from the same individual. The individuals were from four archaeological sites in England: Piddington (1st–2nd centuries AD), Coach Lane, Victoria Gate, and Fewston (all 18th–19th centuries). A method that identifies sex chromosome-linked isoforms of the peptide amelogenin from human tooth enamel was applied. The method utilizes a minimally destructive acid etching procedure and subsequent nano liquid chromatography tandem mass spectrometry. Results: It was possible to determine the sex of 28 of the nonadult individuals sam�pled (males = 20, females = 8, undetermined = 1). Only one sample failed (CL9), due to insufficient mineralization of the sampled tooth enamel. Data are available via ProteomeXchange with identifier PXD021683. Discussion: Sufficient peptide material to determine sex can be recovered even from the crowns of developing perinatal teeth that are not fully mineralized. The minimally destructive and inexpensive (compared to ancient DNA) nature of this procedure has significant implications for bioarchaeological studies of infancy and childhood.

Item Type:Article
Divisions:Science > School of Archaeology, Geography and Environmental Science > Scientific Archaeology
Science > School of Archaeology, Geography and Environmental Science > Department of Archaeology
ID Code:98374


Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation