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Heat-shock response in Arabidopsis thaliana explored by multiplexed quantitative proteomics using differential metabolic labeling

Palmblad, M., Mills, D. J. and Bindschedler, L. V. (2008) Heat-shock response in Arabidopsis thaliana explored by multiplexed quantitative proteomics using differential metabolic labeling. Journal of Proteome Research, 7 (2). pp. 780-785. ISSN 1535-3893

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To link to this item DOI: 10.1021/pr0705340

Abstract/Summary

We have developed a general method for multiplexed quantitative proteomics using differential metabolic stable isotope labeling and mass spectrometry. The method was successfully used to study the dynamics of heat-shock response in Arabidopsis thaliana. A number of known heat-shock proteins were confirmed, and some proteins not previously associated with heat shock were discovered. The method is applicable in stable isotope labeling and allows for high degrees of multiplexing.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
Life Sciences > School of Biological Sciences
ID Code:9870
Uncontrolled Keywords:liquid chromatography, mass spectrometry, quantitation, multiplexing, n-15, stable isotope, metabolic labeling, arabidopsis thaliana, heat shock, coded affinity tags, malic enzyme gene, statistical-model, proteins, stress, expression, family, immunophilins, plants, ms/ms

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