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Rat neurosphere cells protect axotomized rat retinal ganglion cells and facilitate their regeneration

Hill, A.J., Zwart, I., Samaranayake, A.N., Al-Allaf, F., Girdlestone, J., Mehmet, H., Navarrete, R., Navarrete, C. and Jen, L.S. (2009) Rat neurosphere cells protect axotomized rat retinal ganglion cells and facilitate their regeneration. Journal of Neurotrauma, 26 (7). pp. 1147-1156. ISSN 0897-7151

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To link to this article DOI: 10.1089/neu.2008.0801

Abstract/Summary

We investigated the ability of a population of rat neural stem and precursor cells derived from rat embryonic spinal cord to protect injured neurons in the rat central nervous system (CNS). The neonatal rat optic pathway was used as a model of CNS injury, whereby retinal ganglion cells (RGCs) were axotomized by lesion of the lateral geniculate nucleus one day after birth. Neural stem and precursor cells derived from expanded neurospheres (NS) were transplanted into the lesion site at the time of injury. Application of Fast Blue tracer dye to the lesion site demonstrated that significant numbers of RGCs survived at 4 and 8 weeks in animals that received a transplant, with an average of 28% survival, though in some individual cases survival was greater than 50%. No RGCs survived in animals that received a lesion alone. Furthermore, labeled RGCs were also observed when Fast Blue was applied to the superior colliculus (SC) at 4 weeks, suggesting that neurosphere cells also facilitated RGC to regenerate to their normal target. Transplanted cells did not migrate or express neural markers after transplantation, and secreted several neurotrophic factors in vitro. We conclude that NS cells can protect injured CNS neurons and promote their regeneration. These effects are not attributable to cell replacement, and may be mediated via secretion of neurotrophic factors. Thus, neuroprotection by stem cell populations may be a more viable approach for treatment of CNS disorders than cell replacement therapy.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy
ID Code:13732
Uncontrolled Keywords:axonal injury, models of injury, neuronal cell death, stem cells, transplantation, NEURAL STEM-CELLS, SPINAL-CORD-INJURY, LOADED BIODEGRADABLE, MICROSPHERES, PARKINSONS-DISEASE, INTRAVITREAL INJECTIONS, NEUROTROPHIC, FACTORS, MOUSE MODEL, ADULT-RATS, TRANSPLANTATION, NEUROPROTECTION

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