Accessibility navigation


Structure of the DNA octanucleotide d(ACGTACGT)2

Wilcock, D. J., Adams, A., Cardin, C. J. ORCID: https://orcid.org/0000-0002-2556-9995 and Wakelin, L. P. G. (1996) Structure of the DNA octanucleotide d(ACGTACGT)2. Acta Crystallographica Section D - Biological Crystallography, 52 (3). pp. 481-485. ISSN 1399-0047

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1107/S0907444995016052

Abstract/Summary

d(ACGTACGT), C78H84N30O32P7.20H2O, Mr (DNA) = 2170, tetragonal, P43212 (No 96), a = 42.845 (1), b = 42.845(1), c = 24.804 (1) Å, V = 45532.5 (2) Å3, z = 8,(MoK) = 0.71069 Å,µ(MoK) = 0.10 mm-1, T = 295 K, R = 0.18 for 1994 unique reflections between 5.0 and 1.9 Å resolution. The self-complementary octanucleotide d(ACGTACGT)2 has been crystallized and its structure determined to a resolution of 1.9 Å. The asymmetric unit consists of a single strand of octamer with 20 water molecules. It is only the second example of an octanucleotide having terminal A·T base pairs whose structure has been determined by X-ray crystallography. The sequence adopts the modified A-type conformation found for all octanucleotide duplexes studied to date with the helix bent by approximately 15° and an average tilt angle of 0°. Unusually the data collection was carried out using a 3 kW molybdenum sealed-tube source. The conformational details are discussed in comparison with other closely related sequences.

Item Type:Article
Refereed:Yes
Divisions:Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:23771
Publisher:Wiley

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation