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Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity

Lee, S.E., Sidorov, A., Gourlain, T., Mignet, N., Thorpe, S.J., Brazier, J.A., Dickman, M.J., Hornby, D.P., Grasby, J.A. and Williams, D.M. (2001) Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity. Nucleic Acids Research, 29 (7). pp. 1565-1573. ISSN 1362-4962

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To link to this article DOI: 10.1093/nar/29.7.1565

Abstract/Summary

The incorporation of potentially catalytic groups in DNA is of interest for the in vitro selection of novel deoxyribozymes, A series of 10 C5-modified analogues of 2'-deoxyuridine triphosphate have been synthesised that possess side chains of differing flexibility and bearing a primary amino or imidazole functionality, For each series of nucleotide analogues differing degrees of flexibility of the C5 side chain was achieved through the use of alkynyl, alkenyl and alkyl moieties, The imidazole function was conjugated to these CS-amino-modified nucleotides using either imidazole 4-acetic acid or imidazole 4-acrylic acid (urocanic acid), The substrate properties of the nucleotides (fully replacing dTTP) with Taq polymerase during PCR have been investigated in order to evaluate their potential applications for in vitro selection experiments, 5-(3-Aminopropynyl)dUTP and 5-(E-3-aminopropenyl)dUTP and their imidazole 4-acetic acid- and urocanic acid-modified conjugates were found to be substrates, In contrast, C5-amino-modified dUTPs with alkane or Z-alkene linkers and their corresponding conjugates were not substrates, The incorporation of these analogues during PCR has been confirmed by inhibition of restriction enzyme digestion using XbaI and by mass spectrometry of the PCR products.

Item Type:Article
Refereed:Yes
Divisions:No Reading authors. Back catalogue items
ID Code:27310
Uncontrolled Keywords:In-vitro selection; dna-polymerases; bond formation; active-site; rna; nucleosides; nucleotide; ribozyme; 5'-triphosphates; carboxyamidation
Publisher:Oxford University Press

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