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Metal bioaccumulation and cellular fractionation in an epigeic earthworm (Lumbricus rubellus): the interactive influences of population exposure histories, site-specific geochemistry and mitochondrial genotype

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Andre, J., Stürzenbaum, S. R., Kille, P., Morgan, A. J. and Hodson, M. E. (2010) Metal bioaccumulation and cellular fractionation in an epigeic earthworm (Lumbricus rubellus): the interactive influences of population exposure histories, site-specific geochemistry and mitochondrial genotype. Soil Biology & Biochemistry, 42 (9). pp. 1566-1573. ISSN 0038-0717

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To link to this article DOI: 10.1016/j.soilbio.2010.05.029

Abstract/Summary

Subcellular fractionation techniques were used to describe temporal changes (at intervals from T0 to T70 days) in the Pb, Zn and P partitioning profiles of Lumbricus rubellus populations from one calcareous (MDH) and one acidic (MCS) geographically isolated Pb/Zn-mine sites and one reference site (CPF). MDH and MCS individuals were laboratory maintained on their native field soils; CPF worms were exposed to both MDH and MCS soils. Site-specific differences in metal partitioning were found: notably, the putatively metal-adapted populations, MDH and MCS, preferentially partitioned higher proportions of their accumulated tissue metal burdens into insoluble CaPO4-rich organelles compared with naive counterparts, CPF. Thus, it is plausible that efficient metal immobilization is a phenotypic trait characterising metal tolerant ecotypes. Mitochondrial cytochrome oxidase II (COII) genotyping revealed that the populations indigenous to mine and reference soils belong to distinct genetic lineages, differentiated by 13%, with 7 haplotypes within the reference site lineage but fewer (3 and 4, respectively) in the lineage common to the two mine sites. Collectively, these observations raise the possibility that site-related genotype differences could influence the toxico-availability of metals and, thus, represent a potential confounding variable in field-based eco-toxicological assessments.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Science > School of Archaeology, Geography and Environmental Science > Department of Geography and Environmental Science
Interdisciplinary centres and themes > Soil Research Centre
Faculty of Science > School of Archaeology, Geography and Environmental Science > Earth Systems Science
ID Code:7686
Uncontrolled Keywords:Earthworms; Pb & Zn; Subcellular fractionation; Field & lab exposures; Genotyping
Publisher:Elsevier

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