Accessibility navigation


Characterization of peptides and their assemblies

Hamley, I. W. ORCID: https://orcid.org/0000-0002-4549-0926 and Castelletto, V. (2020) Characterization of peptides and their assemblies. In: Guler, M. O. (ed.) Peptide-based Biomaterials. Royal Society of Chemistry, pp. 19-46. ISBN 9781788017299

[img] Text - Accepted Version
· Restricted to Repository staff only until 26 November 2023.

2MB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1039/9781839161148-00019

Abstract/Summary

Methods to characterize the conformation and self-assembly or aggregation of peptides and peptide conjugates are reviewed. Spectroscopic methods to probe conformation, including circular dichroism, Fourier-transform infrared, Raman and NMR spectroscopy, are outlined. Aggregation can be detected by fluorescence probe and analytical ultracentrifugation methods, which are discussed. The analysis of structural information on assemblies from X-ray diffraction, light scattering, small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) is considered. Imaging methods are also summarized, including atomic force microscopy (AFM) and electron microscopy (SEM and TEM). Peptide gel mechanical analysis using rheometry is also discussed. Selected examples from the literature are used to highlight the applicability of the methods.

Item Type:Book or Report Section
Refereed:No
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:94690
Publisher:Royal Society of Chemistry

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation