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Survival of a surrogate African swine fever virus-like algal virus in feed matrices using a 23-day commercial United States truck transport model

Palowski, A., Balestreri, C., Urriola, P. E., van de Ligt, J. L. G., Sampedro, F., Dee, S., Shah, A., Yancy, H. F., Shurson, G. C. and Schroeder, D. C. (2022) Survival of a surrogate African swine fever virus-like algal virus in feed matrices using a 23-day commercial United States truck transport model. Frontiers in Microbiology, 13. 1059118. ISSN 1664-302X

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To link to this item DOI: 10.3389/fmicb.2022.1059118


African swine fever virus (ASFV) is a member of the nucleocytoplasmic large DNA viruses (NCLDVs) and is stable in a variety of environments, including animal feed ingredients as shown in previous laboratory experiments and simulations. virus (EhV) is another member of the NCLDVs, which has a restricted host range limited to a species of marine algae called . This algal NCLDV has many similar morphological and physical characteristics to ASFV thereby making it a safe surrogate, with results that are applicable to ASFV and suitable for use in real-world experiments. Here we inoculated conventional soybean meal (SBMC), organic soybean meal (SBMO), and swine complete feed ( ) matrices with EhV strain 86 (EhV-86) at a concentration of 6.6 × 10 virus g , and then transported these samples in the trailer of a commercial transport vehicle for 23 days across 10,183 km covering 29 states in various regions of the United States. Upon return, samples were evaluated for virus presence and viability using a previously validated viability qPCR (V-qPCR) method. Results showed that EhV-86 was detected in all matrices and no degradation in EhV-86 viability was observed after the 23-day transportation event. Additionally, sampling sensitivity (we recorded unexpected increases, as high as 49% in one matrix, when virus was recovered at the end of the sampling period) rather than virus degradation best explains the variation of virus quantity observed after the 23-day transport simulation. These results demonstrate for the first time that ASFV-like NCLDVs can retain viability in swine feed matrices during long-term transport across the continental United States.

Item Type:Article
Divisions:Life Sciences > School of Biological Sciences > Biomedical Sciences
ID Code:109732
Uncontrolled Keywords:feed, transport, viability PCR, NCLDVs, Emiliania huxleyi virus, African swine fever virus


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