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Effect of glycosylation on self-assembly of lipid A lipopolysaccharides in aqueous solutions

Castelletto, V. ORCID:, Seitsonen, J. and Hamley, I. W. ORCID: (2023) Effect of glycosylation on self-assembly of lipid A lipopolysaccharides in aqueous solutions. Langmuir, 39 (24). pp. 8379-8558. ISSN 0743-7463

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To link to this item DOI: 10.1021/acs.langmuir.3c00828


Lipopolysaccharides (LPSs) based on lipid A produced by bacteria are of interest due to their bioactivity in stimulating immune responses, as are simpler synthetic components or analogues. Here, the self-assembly in water of two monodisperse lipid A derivatives based on simplified bacterial LPS structures is examined and compared to that of a native Escherichia coli LPS using small-angle X-ray scattering and cryogenic transmission electron microscopy. The critical aggregation concentration is obtained from fluorescence probe experiments, and conformation is probed using circular dichroism spectroscopy. The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo2-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively. These observations are rationalized by considering the surfactant packing parameter.

Item Type:Article
Divisions:Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:112234
Publisher:American Chemical Society


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