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Re-pairing DNA: binding of a ruthenium phi complex to a double mismatch

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Prieto, T. D., McQuaid, K. T. ORCID: https://orcid.org/0000-0002-3222-5584, Paterson, N. G., Cardin, D. J., Kellett, A. and Cardin, C. J. ORCID: https://orcid.org/0000-0002-2556-9995 (2024) Re-pairing DNA: binding of a ruthenium phi complex to a double mismatch. Chemical Science, 15 (24). pp. 9096-9103. ISSN 2041-6539 doi: 10.1039/D4SC01448K

Abstract/Summary

We report a crystal structure at atomic resolution (0.9 Å) of a ruthenium complex bound to a consecutive DNA double mismatch, which results in a TA basepair with flipped out thymine, together with the formation of an adenine bulge. The structure shows a form of metalloinsertion interaction of the Λ-[Ru(phen)2phi]2+ (phi = 9,10-phenanthrenediimine) complex at the bulge site. The metal complex interacts with the DNA via the major groove, where specific interactions between the adenines of the DNA and the phen ligands of the complex are formed. One Δ-[Ru(phen)2phi]2+ complex interacts via the minor groove, which shows sandwiching of its phi ligand between the phi ligands of the other two ruthenium complexes, and no interaction of its phen ligands with DNA. To our knowledge, this binding model represents a new form of metalloinsertion in showing major rather than minor groove insertion.

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Item Type Article
URI https://centaur.reading.ac.uk/id/eprint/123034
Identification Number/DOI 10.1039/D4SC01448K
Refereed Yes
Divisions Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Mass Spectrometry (CAF)
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > NMR (CAF)
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Optical Spectroscopy (CAF)
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Xray (CAF)
Publisher Royal Society of Chemistry (RSC)
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