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From Loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2′-deoxyuridine fluorescent probes

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Barr, J., Prieto Otoya, T. D., Cardin, C. and Cadoni, E. (2025) From Loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2′-deoxyuridine fluorescent probes. RSC Chemical Biology. ISSN 2633-0679

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Abstract/Summary

We report the use of 5-furyl-2′-deoxyuridine (5FU) as a fluorescent probe to distinguish ligand binding to distinct G-quadruplex tetrads. Site-specific incorporation of 5FU into T95-2T at the capping regions enabled discrimination of peptide-binding events via fluorescence turn-on, providing insights into tetrad preference and binding affinities using the 5FU-G4/RHAU peptide system.

Item Type:Article
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > NMR (CAF)
ID Code:127282
Publisher:Royal Society of Chemistry
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