From Loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2′-deoxyuridine fluorescent probes

Barr, J., Prieto Otoya, T. D., Cardin, C. and Cadoni, E. (2025) From Loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2′-deoxyuridine fluorescent probes. RSC Chemical Biology. ISSN 2633-0679

Abstract/Summary

We report the use of 5-furyl-2′-deoxyuridine (5FU) as a fluorescent probe to distinguish ligand binding to distinct G-quadruplex tetrads. Site-specific incorporation of 5FU into T95-2T at the capping regions enabled discrimination of peptide-binding events via fluorescence turn-on, providing insights into tetrad preference and binding affinities using the 5FU-G4/RHAU peptide system.

Item Type	Article
URI	https://centaur.reading.ac.uk/id/eprint/127282
Refereed	Yes
Divisions	Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > NMR (CAF)
Publisher	Royal Society of Chemistry
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