Cell cycle–dependent centrosome clustering precedes proplatelet formation

Becker, I. C., Wilkie, A. R., Nikols, E., Carminita, E., Roweth, H. G. ORCID: https://orcid.org/0000-0002-1100-8409, Tilburg, J., Sciaudone, A. R., Noetzli, L. J., Fatima, F., Couldwell, G., Ray, A., Mogilner, A., Machlus, K. R. and Italiano Jr., J. E. (2024) Cell cycle–dependent centrosome clustering precedes proplatelet formation. Science Advances, 10 (25). ISSN 2375-2548 doi: 10.1126/sciadv.adl6153

Abstract/Summary

Platelet-producing megakaryocytes (MKs) primarily reside in the bone marrow, where they duplicate their DNA content with each cell cycle resulting in polyploid cells with an intricate demarcation membrane system. While key elements of the cytoskeletal reorganizations during proplatelet formation have been identified, what initiates the release of platelets into vessel sinusoids remains largely elusive. Using a cell cycle indicator, we observed a unique phenomenon, during which amplified centrosomes in MKs underwent clustering following mitosis, closely followed by proplatelet formation, which exclusively occurred in G1 of interphase. Forced cell cycle arrest in G1 increased proplatelet formation not only in vitro but also in vivo following short-term starvation of mice. We identified that inhibition of the centrosomal protein kinesin family member C1 (KIFC1) impaired clustering and subsequent proplatelet formation, while KIFC1-deficient mice exhibited reduced platelet counts. In summary, we identified KIFC1-and cell cycle–mediated centrosome clustering as an important initiator of proplatelet formation from MKs.

Item Type	Article
URI	https://centaur.reading.ac.uk/id/eprint/127722
Identification Number/DOI	10.1126/sciadv.adl6153
Refereed	Yes
Divisions	No Reading authors. Back catalogue items Life Sciences > School of Biological Sciences > Biomedical Sciences
Publisher	American Association for the Advancement of Science
Download/View statistics	View download statistics for this item