Oxidation affects the flow-induced aggregation of low density lipoprotein and its inhibition by albumin
Talbot, R. M., del Rio, J. D., Leake, D. S. and Weinberg, P. D. (2003) Oxidation affects the flow-induced aggregation of low density lipoprotein and its inhibition by albumin. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids, 1634 (1-2). pp. 24-29. ISSN 1388-1981
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To link to this article DOI: 10.1016/j.bbalip.2003.08.002
We investigated whether oxidation alters the self-aggregation of low density lipoprotein (LDL) and the inhibition of such aggregation by albumin. Incubation with copper for different durations produced mildly, moderately, and highly oxidised LDL (having, respectively, ca. 60, 300 and 160 nmol lipid hydroperoxides/mg protein, and electrophoretic mobilities 1.2, 2.6 and 4.4 times that of native LDL). The rate of flow-induced aggregation was the same for native, mildly oxidised and moderately oxidised LDL, but decreased for highly oxidised LDL. The inhibitory effect of albumin (40 mg/ml) on aggregation was reduced by mild oxidation and further reduced by moderate or severe oxidation. The net result of the two effects was that in the presence of albumin, moderately oxidised LDL had the highest rate of aggregation and native the lowest. The reduction in the anti-aggregatory effect of albumin provides a new mechanism by which LDL oxidation might enhance net aggregation in vivo. (C) 2003 Elsevier B.V. All rights reserved.