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In planta proteomics and proteogenomics of the biotrophic barley fungal pathogen blumeria graminis f. sp hordei

Bindschedler, L. V., Burgis, T. A., Mills, D. J. S., Ho, J. T. C., Cramer, R. and Spanu, P. D. (2009) In planta proteomics and proteogenomics of the biotrophic barley fungal pathogen blumeria graminis f. sp hordei. Molecular & Cellular Proteomics, 8 (10). pp. 2368-2381. ISSN 1535-9476

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To link to this article DOI: 10.1074/mcp.M900188-MCP200

Abstract/Summary

To further our understanding of powdery mildew biology during infection, we undertook a systematic shotgun proteomics analysis of the obligate biotroph Blumeria graminis f. sp. hordei at different stages of development in the host. Moreover we used a proteogenomics approach to feed information into the annotation of the newly sequenced genome. We analyzed and compared the proteomes from three stages of development representing different functions during the plant-dependent vegetative life cycle of this fungus. We identified 441 proteins in ungerminated spores, 775 proteins in epiphytic sporulating hyphae, and 47 proteins from haustoria inside barley leaf epidermal cells and used the data to aid annotation of the B. graminis f. sp. hordei genome. We also compared the differences in the protein complement of these key stages. Although confirming some of the previously reported findings and models derived from the analysis of transcriptome dynamics, our results also suggest that the intracellular haustoria are subject to stress possibly as a result of the plant defense strategy, including the production of reactive oxygen species. In addition, a number of small haustorial proteins with a predicted N-terminal signal peptide for secretion were identified in infected tissues: these represent candidate effector proteins that may play a role in controlling host metabolism and immunity. Molecular & Cellular Proteomics 8: 2368-2381, 2009.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
ID Code:11026
Uncontrolled Keywords:FUSARIUM-GRAMINEARUM, GENE-EXPRESSION, HAUSTORIA, VIRULENCE, PROTEINS, ARABIDOPSIS, INFECTION, PROFILES, PATHWAYS, HOMOLOG
Publisher:American Society for Biochemistry and Molecular Biology

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