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Difference gel electrophoresis

Timms, J.F. and Cramer, R. (2008) Difference gel electrophoresis. Proteomics, 8 (23-24). pp. 4886-4897. ISSN 1615-9853

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To link to this article DOI: 10.1002/pmic.200800298

Abstract/Summary

DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent their analytical limitations in areas such as intact protein analysis, (linear) detection over a wide range of protein abundances and, theoretically, applications where extreme sensitivity is needed. Thus, in quantitative proteomics DIGE is usually complementary to MS-based quantitation and has some distinct advantages. This review describes the basics of DIGE and its unique properties and compares it to MS-based methods in quantitative protein expression analysis.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:11667
Uncontrolled Keywords:2-DE; DIGE, Mass spectrometry, Quantitation , PROTEOMIC EXPRESSION ANALYSIS, LUMINAL EPITHELIAL-CELLS, CODED AFFINITY TAGS, 2-D DIGE ANALYSIS, MASS-SPECTROMETRY, PROTEIN EXPRESSION, QUANTITATIVE PROTEOMICS, FLUORESCENT DYES, OXIDATIVE STRESS, SCHIZOSACCHAROMYCES-POMBE

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