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Determination of the total antioxidant activity of fruits and vegetables by a liposome assay

Roberts, W.G. and Gordon, M.H. (2003) Determination of the total antioxidant activity of fruits and vegetables by a liposome assay. Journal of Agricultural and Food Chemistry, 51 (5). pp. 1486-1493. ISSN 0021-8561

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To link to this item DOI: 10.1021/jf025983t

Abstract/Summary

The effects of mixtures of antioxidants on the oxidation of phospholipids have been investigated in large unilamellar liposomes following initiation by 2,2'-azobis(2-aminopropane) dihydrochloride. The lag phase increased linearly with antioxidant concentration. The lag phases of mixtures containing alpha-tocopherol with ascorbic acid showed synergy between the antioxidants, but mixtures of beta-carotene with cc-tocopherol or ascorbic acid were not synergistic. The liposome system was used to investigate the total antioxidant activity of lipid- and water-soluble extracts from 16 samples of fruits, vegetables, and related food products. The water-soluble extracts caused greater increases in lag phase than the lipid-soluble extracts. The lag phase of liposomes containing the water-soluble extracts from fruits and vegetables increased linearly with the total phenolic concentration, with the continental salad extract having the longest lag phase. The lipid-soluble extract from apples caused the largest increase in lag phase of the lipid-soluble extracts. The lag phases of the lipid-soluble and water-soluble extracts of all fruits and vegetables studied were additive, but no synergy was detected. The lag phase of the liposomes containing both the water-soluble and lipid-soluble extracts varied from 611.5 min for the continental salad extracts to 47.5 min for the cauliflower extracts.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13042
Uncontrolled Keywords:liposomes, antioxidants, fruits, vegetables, POTENTIALLY ANTICARCINOGENIC FLAVONOIDS, LOW-DENSITY-LIPOPROTEIN, VITAMIN-C, RELATIVE CONTRIBUTIONS, PHOSPHOLIPID-BILAYERS, PHENOLIC-COMPOUNDS, DIETARY BURDEN, ASCORBIC-ACID, HEART-DISEASE, OXIDATION

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