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Sulfation of genistein alters its antioxidant properties and its effect on platelet aggregation and monocyte and endothelial function

Rimbach, G., Weinberg, P.D., de Pascual-Teresa, S., Alonso, M.G., Ewins, B.A., Turner, R., Minihane, A.M., Botting, N., Fairley, B., Matsugo, S., Uchida, Y. and Cassidy, A. (2004) Sulfation of genistein alters its antioxidant properties and its effect on platelet aggregation and monocyte and endothelial function. Biochimica Et Biophysica Acta-General Subjects, 1670 (3). pp. 229-237. ISSN 0304-4165

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To link to this article DOI: 10.1016/j.bbagen.2003.12.008

Abstract/Summary

Soy isoflavones have been extensively studied because of their possible benefits to human health. Genistein, the major isoflavone aglycone, has received most attention; however, it undergoes extensive metabolism (e.g. conjugation with sulfuric acid) in the gut and liver, which may affect its biological proper-ties. This study investigated the antioxidant activity and free radical-scavenging properties of genistein, genistein-4'-sulfate and genistein-4'-7-disulfate as well as their effect on platelet aggregation and monocyte and endothelial function. Electron spin resonance spectroscopy (ESR) and spin trapping data and other standard antioxidant assays indicated that genistein is a relatively weak antioxidant compared to quercetin and that its sulfated metabolites are even less effective. Furthermore, genistein-4'-sulfate was less potent than genistem, and genistein-4'-7-disulfate even less potent, at inhibiting collagen-induced platelet aggregation, nitric oxide (NO) production by macrophages, and secretion by primary human endothelial cells of monocyte chemoattractant protein 1 (MCP-1), intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1). The current data suggest that sulfation of genistein, with the associated loss of hydroxyl groups, decreases its antioxidant activity and its effect on platelet aggregation, inflammation, cell adhesion and chemotaxis. (C) 2004 Elsevier B.V All rights reserved.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13044
Uncontrolled Keywords:genistein, isoflavone, sulfation, cell adhesion, platelet aggregation, inflammation, RAW 264.7 MACROPHAGES, TNF-ALPHA SECRETION, CHEMOATTRACTANT PROTEIN-1, GENE-EXPRESSION, DAIDZEIN, MICE, ATHEROSCLEROSIS, BIOAVAILABILITY, ISOFLAVONOIDS, CONSUMPTION

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