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Ribosome modulation factor protects Escherichia coli during heat stress, but this may not be dependent on ribosome dimerisation

Niven, G.W. (2004) Ribosome modulation factor protects Escherichia coli during heat stress, but this may not be dependent on ribosome dimerisation. Archives of Microbiology, 182 (1). pp. 60-66. ISSN 0302-8933

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To link to this item DOI: 10.1007/s00203-004-0698-9

Abstract/Summary

The role of ribosome modulation factor (RMF) in protecting heat-stressed Escherichia coli cells was identified by the observation that cultures of a mutant strain lacking functional RMF (HMY15) were highly heat sensitive in stationary phase compared to those of the parent strain (W3110). No difference in heat sensitivity was observed between these strains in exponential phase, during which RMF is not synthesised. Studies by differential scanning calorimetry demonstrated that the ribosomes of stationary-phase cultures of the mutant strain had lower thermal stability than those of the parent strain in stationary phase, or exponential-phase ribosomes. More rapid breakdown of ribosomes in the mutant strain during heating was confirmed by rRNA analysis and sucrose density gradient centrifugation. Analyses of ribosome composition showed that the 100S dimers dissociated more rapidly during heating than 70S particles. While ribosome dimerisation is a consequence of the conformational changes caused by RMF binding, it may not therefore be essential for RMF-mediated ribosome stabilisation.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13119
Uncontrolled Keywords:ribosome stability, ribosome modulation factor, heat stress, ribosome, dimerisation, DIFFERENTIAL SCANNING CALORIMETRY, THERMAL-DENATURATION, RIBONUCLEOPROTEIN PARTICLES, DEFICIENT MUTANT, BACTERIAL-CELLS, FACTOR, RMF, IDENTIFICATION, EXPRESSION, MAGNESIUM, VIABILITY

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