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Dietary vitamin C down-regulates inflammatory gene expression in apoE4 smokers

Majewicz, J., Rimbach, G., Proteggente, A.R., Lodge, J.K., Kraemer, K. and Minihane, A.M. (2005) Dietary vitamin C down-regulates inflammatory gene expression in apoE4 smokers. Biochemical and Biophysical Research Communications, 338 (2). pp. 951-955. ISSN 0006-291X

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To link to this item DOI: 10.1016/j.bbrc.2005.10.029


The deleterious impact of cigarette smoking on cardiovascular health may be in part attributable to a free radical mediated proinflammatory response in circulating monocytes. In the current investigation, the impact of vitamin C supplementation on monocyte gene expression was determined in apoE4 smokers versus non-smokers. A total of 10 smokers and 11 non-smokers consumed 60 mg/day of vitamin C for four weeks and a fasting blood sample was taken at baseline and post-intervention for the determination of plasma vitamin C and monocyte gene expression profiles using cDNA array and real time PCR. In apoE4 smokers, supplementation resulted in a 43% increase in plasma vitamin C concentrations. Furthermore, a number of genes were differentially expressed more than 2-fold in response to treatment, including a downregulation of the proinflammatory mediators tumor necrosis factor (TNF) beta, TNF receptor, neurotrophin-3 growth factor receptor, and monocyte chemoattractant protein I receptor. The study has identified a number of molecular mechanisms underlying the benefit of vitamin C supplementation in smokers. (c) 2005 Elsevier Inc. All rights reserved.

Item Type:Article
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13173
Uncontrolled Keywords:smokers, vitamin C, ApoE genotype, monocytes, inflammation, tumor, necrosis factor beta, monocyte chemoattractant protein 1, tyrosine, receptor kinase-beta, microarray, APOLIPOPROTEIN-E GENOTYPE, CORONARY-HEART-DISEASE, TUMOR-NECROSIS-FACTOR, BLOOD MONONUCLEAR-CELLS, KAPPA-B ACTIVATION, CARDIOVASCULAR-DISEASE, CIGARETTE-SMOKING, TOBACCO-SMOKE, ASCORBIC-ACID, FACTOR-ALPHA

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