Accessibility navigation


Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages

Jofre-Monseny, L., de Pascual-Teresa, S., Plonka, E., Huebbe, P., Boesch-Saadatmandi, C., Minihane, A.M. and Rimbach, G. (2007) Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages. British Journal of Nutrition, 97 (5). pp. 864-871. ISSN 0007-1145

Full text not archived in this repository.

To link to this article DOI: 10.1017/s0007114507669219

Abstract/Summary

ApoE is secreted by macrophages at the lesion site of the atherosclerotic plaque, where it is thought to play a protective role against atherosclerosis independently of its effects on lipid metabolism. Of the three common isoforms for apoE, apoE4 is associated with higher risk of cardiovascular disease (CVD). In vitro studies have shown that recombinant apoE may act as an antioxidant in an isoform-dependent manner (E2 > E3 > E4). The oxidative status of the macrophages plays a key role in the process of atherosclerosis. In the present study the possible differential actions of apoE3 and apoE4 on several parameters of oxidative status were determined in stably transfected murine macrophages (RAW 2647-apoE3 and apoE4). No differences between genotypes were observed after peroxide challenge in either protection against cytotoxicity or in cell membrane oxidation, and modest differences were observed in the non-enzymatic antioxidants (glutathione and a-tocopherol) in apoE3 v. apoE4 macrophages. Importantly, cells secreting apoE4 showed increased membrane oxidation under basal conditions, and produced more NO and superoxide anion radicals than the apoE3 macrophages after stimulation. The present data suggest that apoE genotype influences the oxidative status of macrophages, and this could partly contribute to the higher CVD risk observed in apoE4 carriers.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13273
Uncontrolled Keywords:apoE, macrophage, atherosclerosis, alpha-tocopherol, lipid oxidation, glutathione, nitric oxide, superoxide anion, oxidative stress, NITRIC-OXIDE PRODUCTION, CELL-MEDIATED OXIDATION, CORONARY-HEART-DISEASE, E-DEFICIENT MICE, ALZHEIMERS-DISEASE, VITAMIN-E, ANTIOXIDANT ACTIVITY, LIPID-PEROXIDATION, E GENOTYPE, CHOLESTEROL EFFLUX

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation