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Development of a process for the production and purification of alpha- and beta-galactooligosaccharides from Bifobacterium bifidum NCIMB 41171

Goulas, A., Tzortzis, G. and Gibson, G.R. (2007) Development of a process for the production and purification of alpha- and beta-galactooligosaccharides from Bifobacterium bifidum NCIMB 41171. International Dairy Journal, 17 (6). pp. 648-656. ISSN 0958-6946

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To link to this article DOI: 10.1016/j.idairyj.2006.08.010

Abstract/Summary

Synthesis of prebiotic alpha- and beta-galactooligosaccharides (GOS) using the whole cells of Bifidobacterium bifidum NCIMB 41171 was investigated. Determination of alpha- and beta-galactosidase activities showed them to be at 3 and 205 g(-1) of freeze dried biomass, respectively, and they increased to 5 and 344 U g(-1), respectively, when cells were treated with toluene. Starting with 450-500 mg mL(-1) lactose, maximum GOS concentrations were observed at 80-85% lactose conversions and the mixtures contained oligosaccharides (with a degree of polymerisation >= 3) at 77-109 mg mL(-1) and trans-galactosylated disaccharides between 85-115 mg mL(-1). The GOS yield values varied between 36% and 43%. An alpha-linked disaccharide was detected and its presence was confirmed by gas chromatography mass spectroscopy. Cells were re-used up to 8 times without changes in reaction times or the substrate conversions to GOS. Oligosaccharide synthesis was not inhibited by the presence of glucose or galactose. The mixtures were successfully purified from glucose (92% of glucose removed) by fermentation with Saccharomyces cerevisiae with no losses in the oligosaccharide content and only a small decrease on the galactose. (c) 2006 Elsevier Ltd. All rights reserved.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13359
Uncontrolled Keywords:galactooligosaccharides, prebiotics, Bifidobacterium bifidum, Saccharomyces cerevisiae, purification, FOOD-GRADE OLIGOSACCHARIDES, HUMAN COLONIC MICROBIOTA, GALACTO-OLIGOSACCHARIDES, BIFIDOBACTERIUM-BIFIDUM, ENZYMATIC-SYNTHESIS, DIETARY MODULATION, SKIM MILK, LACTOSE, FERMENTATION, HYDROLYSIS

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