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Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function

Gottstein, N., Ewins, B.A., Eccleston, C., Hubbard, G.P., Kavanagh, I.C., Minihane, A.M., Weinberg, P.D. and Rimbach, G. (2003) Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function. British Journal of Nutrition, 89 (5). pp. 607-615. ISSN 0007-1145

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To link to this article DOI: 10.1079/bjn2003820

Abstract/Summary

There has been much recent interest in the cardiovascular benefits of dietary isoflavones. The aim of the present in vitro studies was to investigate potential anti-thrombogenic and anti-atherogenic effects of the isoflavones genistein and daidzein in platelets, macrophages and endothelial cells. Pre-treatment with either isoflavone inhibited collagen-induced platelet aggregation in a dose-dependent manner. In a macrophage cell line (RAW 264-7) activated with interferon gamma plus lipopolysaccharide, both isoflavones were found to inhibit NO production and tumour necrosis factor alpha (TNF-alpha) secretion dose-dependently, but they did not affect mRNA levels for inducible nitric oxide synthase and cyclo-oxygenase-2. Both isoflavones also dose-dependently decreased monocyte chemoattractant protein-1 secretion induced by TNF-alpha in human umbilical vein endothelial cells. Compared with daidzein, genistein exerted greater inhibitory effects for all parameters studied. The present data contributes to our knowledge on the molecular mechanisms by which isoflavones may protect against coronary artery disease. Further studies are required to determine whether the effects of isoflavones observed in the current in vitro studies are relevant to the aetiology of coronary artery disease in vivo.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13361
Uncontrolled Keywords:genistein, daidzein, platelet aggregation, arteriosclerosis, LOW-DENSITY-LIPOPROTEIN, TNF-ALPHA SECRETION, NITRIC-OXIDE, GENE-EXPRESSION, CHEMOATTRACTANT PROTEIN-1, ATHEROSCLEROTIC LESIONS, ANTIOXIDANT ACTIVITIES, RAW-264.7 MACROPHAGES, ISOFLAVONE GENISTEIN, INTERFERON-GAMMA

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