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Mixed culture fermentation studies on the effects of synbiotics on the human intestinal pathogens Campylobacter jejuni and Escherichia coli

Fooks, L.J. and Gibson, G.R. (2003) Mixed culture fermentation studies on the effects of synbiotics on the human intestinal pathogens Campylobacter jejuni and Escherichia coli. Anaerobe, 9 (5). pp. 231-242. ISSN 1075-9964

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To link to this article DOI: 10.1016/s1075-9964(03)00043-x

Abstract/Summary

Batch and continuous culture anaerobic fermentation systems, inoculated with human faeces, were utilised to investigate the antimicrobial actions of two probiotics, Lactobacillus plantartan 0407, combined with oligofructose and Bifidobacterium bifidum Bb12, combined with a mixture of oligofructose and xylo-oligosaccharides (50:50 w/w) against E coli and Campylobacter jejuni. In batch fermenters, both E coli and C jejuni were inhibited by the synbiotics, even when the culture pH was maintained at around neutral. In continuous culture C jejuni was inhibited but the synbiotic failed to inhibit E coli. Although no definitive answer in addressing the mechanisms underlying antimicrobial activity was derived, results suggested that acetate and lactate directly were conferring antagonistic action, rather than as a result of lowering culture pH. In the course of the study culturing and fluorescent in situ hybridisation (FISH) methodologies for the enumeration of bacterial populations were compared. Bifidobacterial populations were underestimated using plating techniques, suggesting the non-culturability of certain bifidobacterial species. (C) 2003 Elsevier Ltd. All rights reserved.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13416
Uncontrolled Keywords:probiotics, prebiotics, synbiotics, Campylobacter jejuni, Escherichia, coli, ANTIBIOTIC-ASSOCIATED DIARRHEA, CLOSTRIDIUM-DIFFICILE COLITIS, HUMAN, LACTOBACILLUS STRAIN, GG PROMOTES RECOVERY, SACCHAROMYCES-BOULARDII, YOUNG-CHILDREN, PREVENTION, BACTERIA, YOGURT, BIFIDOBACTERIUM

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