Accessibility navigation


Associations between apolipoprotein e genotype and circulating F-2-isoprostane levels in humans

Dietrich, M., Hu, Y.Q., Block, G., Olano, E., Packer, L., Morrow, J.D., Hudes, M., Abdukeyum, G., Rimbach, G. and Minihane, A.M. (2005) Associations between apolipoprotein e genotype and circulating F-2-isoprostane levels in humans. Lipids, 40 (4). pp. 329-334. ISSN 0024-4201

Full text not archived in this repository.

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1007/s11745-006-1390-4

Abstract/Summary

Apolipoprotein E (apoE), an important determinant of plasma lipoprotein metabolism, has three common alleles (ε 2, ε 3, and ε 4). Population studies have shown that the risk of diseases characterized by oxidative damage, such as coronary heart disease and Alzheimer's disease, is significantly higher in ε 4 carriers. We evaluated the association between apoE genotypes and plasma F-2-isoprostane levels, an index of lipid peroxidation, in humans. Two hundred seventy-four healthy subjects (104 males, 170 females; 46.9 ± 13.0 yr; 200 whites, 74 blacks; 81 nonsmokers, 64 passive smokers, and 129 active smokers) recruited for a randomized clinical antioxidant intervention trial were included in this analysis. ApoE genotype was determined by PCR and restriction enzyme digestion. Free plasma F2-isoprostane was measured by GC-MS. Genotype groups were compared using multiple regression analysis with adjustment for sex, age, race, smoking status, body mass index, plasma ascorbic acid, and β-carotene. Subjects with ε 3/ε 4 and ε 4/ε 4 genotype (ε 4-carriers) and with ε 2/ε 3 and ε 3/ε 3 (non-ε 4-carriers) were pooled for analysis. In subjects with high cholesterol levels (total cholesterol above 200 mg/dl), plasma F-2-isoprostane levels were 29% higher in ε 4 carriers than in non-ε 4-carriers (P= 0.0056). High-cholesterol subjects that are ε 4 carriers have significantly higher levels of lipid peroxidation as assessed by circulating F-2-isoprostane levels.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13464
Uncontrolled Keywords:IN-VIVO FORMATION, OXIDATIVE STRESS, LIPID-PEROXIDATION, ALZHEIMERS-DISEASE, E POLYMORPHISM, BIOMARKER F-2-ISOPROSTANES, CARDIOVASCULAR-DISEASE, DENSITY-LIPOPROTEINS, METHIONINE RESIDUES, APOE, POLYMORPHISM

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation