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rRNA probes used to quantify the effects of glycomacropeptide and alpha-lactalbumin supplementation on the predominant groups of intestinal bacteria of infant rhesus monkeys challenged with enteropathogenic Escherichia coli

Bruck, W.M., Kelleher, S.L., Gibson, G.R., Nielsen, K.E., Chatterton, D.E.W. and Lonnerdal, B. (2003) rRNA probes used to quantify the effects of glycomacropeptide and alpha-lactalbumin supplementation on the predominant groups of intestinal bacteria of infant rhesus monkeys challenged with enteropathogenic Escherichia coli. Journal of Pediatric Gastroenterology and Nutrition, 37 (3). pp. 273-280. ISSN 0277-2116

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Abstract/Summary

Objectives: Certain milk factors may help to promote the growth of a host-friendly colonic microflora (e.g. bifidobacteria, lactobacilli) and explain why breast-fed infants experience fewer and milder intestinal infections than those who are formula-fed. The effects of supplementation of formula with two such milk factors was investigated in this study. Materials and Methods: Infant rhesus macaques were breastfed, fed control formula, or formula supplemented with glycomacropeptide (GMP) or alpha-lactalburnin (alpha-LA) from birth to 5 months of age. Blood was drawn monthly and rectal swabs were collected weekly. At 4.5 months of age, 10(8) colonyforming units of enteropathogenic E.coli O127, strain 2349/68 (EPEC) was given orally and the response to infection assessed. The bacteriology of rectal swabs pre- and post-infection was determined by culture independent fluorescence in situ hybridization. Results: Post-challenge, breast-fed infants and infants fed alpha-LA-supplemented formula had no diarrhea, whilst those infants fed GMP-supplemented formula had intermittent diarrhea. In infants fed control formula the diarrhea was acute. Conclusions: Supplementation of infant formula with appropriate milk proteins may be useful for improving the infant's ability to resist acute infection caused by E.coli.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13533
Uncontrolled Keywords:microbial and immune mediated injury/pathology, clinical nutrition, development of gastrointestinal function, host defense mechanisms, disorders of gut development, IN-SITU HYBRIDIZATION, KAPPA-CASEIN GLYCOMACROPEPTIDE, HUMAN-MILK, OLIGONUCLEOTIDE PROBES, FUNCTIONAL FOOD, SIALIC-ACID, STRAINS, BIFIDOBACTERIUM, INHIBITION, FRACTIONS

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