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Genistein affects the expression of genes involved in blood pressure regulation and angiogenesis in primary human endothelial cells

Ambra, R., Rimbach, G., Teresa, S.D., Fuchs, D., Wenzel, U., Daniel, H. and Virgili, F. (2006) Genistein affects the expression of genes involved in blood pressure regulation and angiogenesis in primary human endothelial cells. Nutrition Metabolism and Cardiovascular Diseases, 16 (1). pp. 35-43. ISSN 0939-4753

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To link to this item DOI: 10.1016/j.numecd.2005.03.003

Abstract/Summary

Background: Several lines of evidence suggest that the dietary isoflavone genistein (Gen) has beneficial effects with regard to cardiovascular disease and in particular on aspects related to blood pressure and angiogenesis. The biological action of Gen may be, at Least in part, attributed to its ability to affect cell signalling and response. However, so far, most of the molecular mechanisms underlying the activity of Gen in the endothelium are unknown. Methods and results: To examine the transcriptional response to 2.5 mu M Gen on primary human endothelial cells (HUVEC), we applied cDNA array technology both under baseline condition and after treatment with the pro-atherogenic stimulus, copper-oxidized LDL. The alteration of the expression patterns of individual transcripts was substantiated using either RT-PCR or Northern blotting. Gen significantly affected the expression of genes encoding for proteins centrally involved in the vascular tone such as endothelin-converting enzyme-1, endothetin-2, estrogen related receptor a and atria[ natriuretic peptide receptor A precursor. Furthermore, Gen countered the effect of oxLDL on mRNA levels encoding for vascular endothelial growth factor receptor 165, types 1 and 2. Conclusions: Our data indicate that physiologically achievable levels of Gen change the expression of mRNA encoding for proteins involved in the control of blood pressure under baseline conditions and reduce the angiogenic response to oxLDL in the endothelium. (c) 2005 Elsevier B.V. All rights reserved.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences
ID Code:13581
Uncontrolled Keywords:genistein, endothelial cells, gene expression, blood pressure, angiogenesis, ESSENTIAL-HYPERTENSION, PERIMENOPAUSAL WOMEN, PLATELET-AGGREGATION, PLASMA, LIPOPROTEINS, ISOFLAVONES, MENOPAUSAL, INHIBITORS, CARCINOMA, PEPTIDES

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