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Regulation of cell cycle and stress responses to hydrostatic pressure in fission yeast

George, V.T., Brooks, G. and Humphrey, T.C. (2007) Regulation of cell cycle and stress responses to hydrostatic pressure in fission yeast. Molecular Biology of the Cel, 18 (10). pp. 4168-4179. ISSN 1059-1524

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To link to this article DOI: 10.1091/mbc.E06-12-1141

Abstract/Summary

We have investigated the cellular responses to hydrostatic pressure by using the fission yeast Schizosaccharomyces pombe as a model system. Exposure to sublethal levels of hydrostatic pressure resulted in G2 cell cycle delay. This delay resulted from Cdc2 tyrosine-15 (Y-15) phosphorylation, and it was abrogated by simultaneous disruption of the Cdc2 kinase regulators Cdc25 and Wee1. However, cell cycle delay was independent of the DNA damage, cytokinesis, and cell size checkpoints, suggesting a novel mechanism of Cdc2-Y15 phosphorylation in response to hydrostatic pressure. Spc1/Sty1 mitogen-activated protein (MAP) kinase, a conserved member of the eukaryotic stress-activated p38, mitogen-activated protein (MAP) kinase family, was rapidly activated after pressure stress, and it was required for cell cycle recovery under these conditions, in part through promoting polo kinase (Plo1) phosphorylation on serine 402. Moreover, the Spc1 MAP kinase pathway played a key role in maintaining cell viability under hydrostatic pressure stress through the bZip transcription factor, Atf1. Further analysis revealed that prestressing cells with heat increased barotolerance, suggesting adaptational cross-talk between these stress responses. These findings provide new insight into eukaryotic homeostasis after exposure to pressure stress.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Biological Sciences > Biomedical Sciences
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy
Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
ID Code:13743
Uncontrolled Keywords:ACTIVATED PROTEIN-KINASE, ATF1 TRANSCRIPTION FACTOR, MAP KINASE, SCHIZOSACCHAROMYCES-POMBE, SACCHAROMYCES-CEREVISIAE, CDC25 PHOSPHATASE, OXIDATIVE STRESS, COUPLING MITOSIS, DIRECT INDUCTION, DNA-REPAIR
Publisher:American Society for Cell Biology

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