Accessibility navigation


First-dimension separation with the MicroRotoforTM cell prior to SDS-PAGE and LC-MS/MS analysis

Bindschedler, L. V., Palmblad, M. and Cramer, R. K. (2006) First-dimension separation with the MicroRotoforTM cell prior to SDS-PAGE and LC-MS/MS analysis. Bio-Rad Bulletin, 5451. pp. 1-4.

Full text not archived in this repository.

Official URL: http://www3.bio-rad.com/

Abstract/Summary

Free-flow isoelectric focusing (IEF) is a gel-free method for separating proteins based on their isoelectric point (pl) in a liquid environment and in the presence of carrier ampholytes. this method has been used with the RotoforTM cell at the preparative scale to fractionate proteins from samples containing several hundred milligrams of protein; see the refeences listed in Bio-Rad bulletin 3152. the MicroRotofor cell applies the same method to much sl=maller protein samples without dilution, separating and recoverng milligram quantities of protein in a total volume of about 2 ml.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
ID Code:16346

Centaur Editors: Update this record

Page navigation