Accessibility navigation


Tissue engineering a fetal membrane

Downloads

Downloads per month over past year

Mi, S., David, A. L., Chowdhury, B., Jones, R. R., Hamley, I., Squires, A. and Connon, C. J. (2012) Tissue engineering a fetal membrane. Tissue Engineering Part A, 18 (3-4). pp. 373-381. ISSN 2152-4955

[img] Text - Published Version
· Please see our End User Agreement before downloading.

782Kb

To link to this article DOI: 10.1089/ten.tea.2011.0194

Abstract/Summary

The aim of this study was to construct an artificial fetal membrane (FM) by combination of human amniotic epithelial stem cells (hAESCs) and a mechanically enhanced collagen scaffold containing encapsulated human amniotic stromal fibroblasts (hASFs). Such a tissue-engineered FM may have the potential to plug structural defects in the amniotic sac after antenatal interventions, or to prevent preterm premature rupture of the FM. The hAESCs and hASFs were isolated from human fetal amniotic membrane (AM). Magnetic cell sorting was used to enrich the hAESCs by positive ATP-binding cassette G2 selection. We investigated the use of a laminin/fibronectin (1:1)-coated compressed collagen gel as a novel scaffold to support the growth of hAESCs. A type I collagen gel was dehydrated to form a material mimicking the mechanical properties and ultra-structure of human AM. hAESCs successfully adhered to and formed a monolayer upon the biomimetic collagen scaffold. The resulting artificial membrane shared a high degree of similarity in cell morphology, protein expression profiles, and structure to normal fetal AM. This study provides the first line of evidence that a compacted collagen gel containing hASFs could adequately support hAESCs adhesion and differentiation to a degree that is comparable to the normal human fetal AM in terms of structure and maintenance of cell phenotype.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Electron Microscopy Laboratory (CAF)
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:24314
Publisher:Mary Ann Liebert, Inc.
Publisher Statement:This is a copy of an article published in the journal Tissue Engineering Part A © 2012[copyright Mary Ann Liebert, Inc.]; Tissue Engineering Part A is available online at: http://www.liebertonline.com

Download Statistics for this item.

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation