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A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film

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Edwards, A. D., Reis, N. M., Slater, N. K. H. and Mackley, M. R. (2011) A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film. Lab on a Chip, 11 (24). pp. 4267-4273. ISSN 1473-0197

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To link to this article DOI: 10.1039/C0LC00357C

Abstract/Summary

We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200µm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
ID Code:24356
Publisher:Royal Society of Chemistry

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