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Application of DIGE and mass spectrometry in the study of type 2 Diabetes Mellitus (T2DM) mouse models

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Smith, C., Mills, D. and Cramer, R. (2012) Application of DIGE and mass spectrometry in the study of type 2 Diabetes Mellitus (T2DM) mouse models. In: Cramer, R. and Westermeier , R. (eds.) Difference Gel Electrophoresis (DIGE). Methods in molecular biology (854). Humana Press / Springer, New York, pp. 299-318. ISBN 9781617795725

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To link to this item DOI: 10.1007/978-1-61779-573-2_21

Abstract/Summary

Knowledge of the differences between the amounts and types of protein that are expressed in diseased compared to healthy subjects may give an understanding of the biological pathways that cause disease. This is the reasoning behind the presented protocol, which uses difference gel electrophoresis to discover up‐ or down‐regulated proteins between mice of different genotypes, or of those fed on different diets, that may thus be prone to develop diabetes‐like phenotypes. Subsequent analysis of these proteins by tandem mass spectrometry typically facilitates their identification with a high degree of confidence.

Item Type:Book or Report Section
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Chemical Analysis Facility (CAF)
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry
ID Code:30234
Publisher:Humana Press / Springer

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