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Gap junction-mediated spontaneous Ca(2+) waves in differentiated cholinergic SN56 cells

Hettiarachchi, N. T., Dallas, M. L., Pearson, H. A., Bruce, G., Deuchars, S., Boyle, J. P. and Peers, C. (2010) Gap junction-mediated spontaneous Ca(2+) waves in differentiated cholinergic SN56 cells. Biochemical and Biophysical Research Communications, 397 (3). pp. 564-568. ISSN 0006-291X

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To link to this item DOI: 10.1016/j.bbrc.2010.05.159

Abstract/Summary

Neuronal gap junctions are receiving increasing attention as a physiological means of intercellular communication, yet our understanding of them is poorly developed when compared to synaptic communication. Using microfluorimetry, we demonstrate that differentiation of SN56 cells (hybridoma cells derived from murine septal neurones) leads to the spontaneous generation of Ca(2+) waves. These waves were unaffected by tetrodotoxin (1microM), but blocked by removal of extracellular Ca(2+), or addition of non-specific Ca(2+) channel inhibitors (Cd(2+) (0.1mM) or Ni(2+) (1mM)). Combined application of antagonists of NMDA receptors (AP5; 100microM), AMPA/kainate receptors (NBQX; 20microM), nicotinic AChR receptors (hexamethonium; 100microM) or inotropic purinoceptors (brilliant blue; 100nM) was also without effect. However, Ca(2+) waves were fully prevented by carbenoxolone (200microM), halothane (3mM) or niflumic acid (100microM), three structurally diverse inhibitors of gap junctions, and mRNA for connexin 36 was detected by PCR. Whole-cell patch-clamp recordings revealed spontaneous inward currents in voltage-clamped cells which we inhibited by Cd(2+), Ni(2+) or niflumic acid. Our data suggest that differentiated SN56 cells generated spontaneous Ca(2+) waves which are propagated by intercellular gap junctions. We propose that this system can be exploited conveniently for the development of neuronal gap junction modulators.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy
No Reading authors. Back catalogue items
ID Code:30342
Uncontrolled Keywords:Calcium oscillation; Gap junction; Cholinergic; Patch clamp
Publisher:Elsevier

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