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The effect of proteolysis on the induction of cell death by monomeric alpha-lactalbumin.

Bruck, W. M., Gibson, G. R. and Bruck, T. B. (2014) The effect of proteolysis on the induction of cell death by monomeric alpha-lactalbumin. Biochimie, 97. pp. 138-143. ISSN 0300-9084

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To link to this item DOI: 10.1016/j.biochi.2013.10.007

Abstract/Summary

α-Lactalbumin (α-la) is a major whey protein found in milk. Previous data suggested that α-la has antiproliferative effects in human adenocarcinoma cell lines such as Caco-2 and HT-29. However, the cell death inducing α-la was not a naturally occurring monomer but either a multimeric variant or an α-la:oleic acid complex (HAMLET/BAMLET). Proteolysis showed that both human and bovine α-la are susceptible to digestion. ELISA assays assessing cell death with the native undigested α-la fractions showed that undigested protein fractions did have a significant cell death effect on CaCo-2 cells. Bovine α-la was also more effective than human α-la. A reduction in activity corresponded with lower concentrations of the protein and partial digestion and fragmentation of the protein using trypsin and pepsin. This suggests that the tertiary structure is vital for the apoptotic effect.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
ID Code:35767
Publisher:Elsevier

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