Accessibility navigation


Lab on a stick: multi-analyte cellular assays in a microfluidic dipstick

Reis, N. M., Pivetal, J., Loo-Zazueta, A. L., Barros, J. M. S. and Edwards, A. D. ORCID: https://orcid.org/0000-0003-2369-989X (2016) Lab on a stick: multi-analyte cellular assays in a microfluidic dipstick. Lab on a Chip, 16 (15). pp. 2891-2899. ISSN 1473-0189

[img]
Preview
Text (Open Access) - Published Version
· Available under License Creative Commons Attribution.
· Please see our End User Agreement before downloading.

4MB
[img]
Preview
Text - Accepted Version
· Please see our End User Agreement before downloading.

1MB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1039/C6LC00332J

Abstract/Summary

A new microfluidic concept for multi-analyte testing in a dipstick format is presented, termed “Lab-on-a-Stick”, that combines the simplicity of dipstick tests with the high performance of microfluidic devices. Lab-on-a-Stick tests are ideally suited to analysis of particulate samples such as mammalian or bacterial cells, and capable of performing multiple different parallel microfluidic assays when dipped into a single sample with results recorded optically. The utility of this new diagnostics format was demonstrated by performing three types of multiplex cellular assays that are challenging to perform in conventional dipsticks: 1) instantaneous ABO blood typing; 2) microbial identification; and 3) antibiotic minimum inhibitory (MIC) concentration measurement. A pressure balance model closely predicted the superficial flow velocities in individual capillaries, that were overestimated by up to one order of magnitude by the Lucas-Washburn equation conventionally used for wicking in cylindrical pores. Lab-on-a-stick provides a cost-effective, simple, portable and flexible multiplex platform for a range of assays, and will deliver a new generation of advanced yet affordable point-of-care tests for global diagnostics.

Item Type:Article
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Chemical Analysis Facility (CAF) > Mass Spectrometry (CAF)
Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Pharmaceutics Research Group
ID Code:66028
Publisher:Royal Society of Chemistry

Downloads

Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation