Accessibility navigation


Development and characterisation of a novel NF-κB reporter cell line for investigation of neuroinflammation

Zeuner, M.-T., Vallance, T., Vaiyapuri, S., Cottrell, G. S. and Widera, D. (2017) Development and characterisation of a novel NF-κB reporter cell line for investigation of neuroinflammation. Mediators of inflammation, 2017. 6209865. ISSN 0962-9351

[img] Text (Open Access) - Published Version
· Available under License Creative Commons Attribution.
· Please see our End User Agreement before downloading.

2MB
[img] Text - Accepted Version
· Restricted to Repository staff only

643kB

It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing.

To link to this item DOI: 10.1155/2017/6209865

Abstract/Summary

Aberrant activation of the transcription factor NF-κB, as well as uncontrolled inflammation has been linked to autoimmune diseases, development and progression of cancer and neurological disorders like Alzheimer’s disease. Reporter cell lines are a valuable state-of-the art tool for comparative analysis of in vitro drug screening. However, a reporter cell line for the investigation of NF-κB-driven neuroinflammation has not yet been available. Thus, we developed a stable neural NF-κB-reporter cell line to assess the potency of pro-inflammatory molecules and peptides, as well as anti-inflammatory pharmaceuticals. We used lentivirus to transduce the glioma cell line U251-MG with a tandem NF-κB reporter construct containing GFP and firefly luciferase allowing an assessment of NF-κB activity via fluorescence microscopy, flow cytometry and luminometry. We observed a robust activation of NF-κB after exposure of the reporter cell line to Tumour 2 necrosis factor alpha (TNFα) and amyloid-β peptide [1-42] as well as to LPS derived from Salmonella minnesota and Escherichia coli. Finally, we demonstrate that the U251-NF-κB-GFP-Luc reporter cells can be used for assessing the anti-inflammatory potential of pharmaceutical compounds using Bay11-7082 and IMD0354. In summary, our newly generated cell line is a robust and cost-efficient tool to study pro- and anti-inflammatory potential of drugs and biologicals in neural cells.

Item Type:Article
Refereed:Yes
Divisions:Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy > Division of Pharmacology
ID Code:70869
Publisher:Hindawi Publishing Corporation

Downloads

Downloads per month over past year

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation