Accessibility navigation


Ultrasound processing of liquid system(s) and its antimicrobial mechanism of action

Downloads

Downloads per month over past year

Spiteri, D., Claire , C.-P., Sclear, J. , Karatzas, K. A., Scerri, C. and Valdramidis, V. (2017) Ultrasound processing of liquid system(s) and its antimicrobial mechanism of action. Letters in Applied Microbiology. ISSN 0266-8254 (In Press)

[img] Text - Accepted Version
· Restricted to Repository staff only
· The Copyright of this document has not been checked yet. This may affect its availability.

348Kb

To link to this item DOI: 10.1111/lam.12776

Abstract/Summary

Ultrasound creates cavitation phenomena, resulting in the formation of several free radicals, namely OH and H, due to the breakdown of the H2O molecule. These radicals affect the cellular integrity of the bacteria, causing the inactivation of several processes, and thus it is important to unravel the mechanism of action of this technology. This research looks into the application and mechanism of action of ultrasound technology as a means of disinfection by acoustic cavitation. Sterile water and synthetic waste water were inoculated with different mutants of E. coli K12 strains containing deletions in genes affecting specific functional properties of E. coli. These were: dnak soxR, soxS, oxyR, rpoS, gadA/gadB, gadC and yneL. E. coli K-12 ΔoxyR, appeared to be more resistant to the treatment together with gadW, gadX, gabT and gabD, whereas the mutant K-12 ΔdnaK was more sensitive with approximately 2.5 log (CFU/mL) reduction in comparison to their isogenic wild type E. coli K-12. This indicates that the dnaK gene participates in general stress response and more specifically to hyperosmotic stress. The other E. coli deleted genes tested (soxS, rpoS, gadB, gadC, yneL) did not appear to be involved in protection of microbial cells against ultrasound

Item Type:Article
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Centre for Integrative Neuroscience and Neurodynamics (CINN)
Faculty of Life Sciences > School of Chemistry, Food and Pharmacy > Department of Food and Nutritional Sciences > Food Microbial Sciences Research Group
ID Code:71448
Publisher:Wiley

University Staff: Request a correction | Centaur Editors: Update this record

Page navigation