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The study of platelet receptors using artificial lipid bilayers

Dustin, M. L. and Pollitt, A. Y. (2018) The study of platelet receptors using artificial lipid bilayers. In: Gibbins, J. M. and Mahaut-Smith, M. (eds.) Platelets and Megakaryocytes: Volume 4, Advanced Protocols and Perspectives. Methods in Molecular Biology, 1812. Humana Press, New York, pp. 127-137. ISBN 9781493985845

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To link to this item DOI: 10.1007/978-1-4939-8585-2_8

Abstract/Summary

Artificial lipid bilayers are powerful tools that can be used to model the interactions between platelets and membrane-bound ligands. To mimic the interaction of platelets with membrane-bound ligands, biotinylated lipids can be used to couple monobiotinylated recombinant ligands to the upper leaflet of an artificial lipid bilayer using streptavidin to bridge the two. Artificial lipid bilayers are generated by preparing liposomes, treating glass coverslips to make them hydrophilic and by assembling the bilayer in a specialized flow chamber. Finally platelets can be added to the flow chamber and the localization of fluorescently labeled molecules followed using microscopy.

Item Type:Book or Report Section
Refereed:Yes
Divisions:Interdisciplinary centres and themes > Institute for Cardiovascular and Metabolic Research (ICMR)
ID Code:79198
Uncontrolled Keywords:Artificial lipid bilayer, CLEC-2, Podoplanin, Receptor–ligand interactions
Publisher:Humana Press

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