Liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry using a commercial ion source and orbitrap mass analyzer
Challen, B.
It is advisable to refer to the publisher's version if you intend to cite from this work. See Guidance on citing. To link to this item DOI: 10.1021/acs.analchem.4c04458 Abstract/SummaryA liquid atmospheric pressure-matrix-assisted laser desorption/ionization (LAP-MALDI) method has been developed and applied to a commercial AP-MALDI source on a hybrid orbitrap mass analyzer. It is shown that electrospray ionization (ESI)-like mass spectra of a range of peptides and proteins can be acquired by LAP-MALDI mass spectrometry (MS) as previously demonstrated on a home-made LAP-MALDI-Q-TOF setup but without the need of any modification to the commercially available MS equipment used. Multiply charged peptide ions were recorded with a resolution of around 100,000 and a mass accuracy of less than 5 ppm. The higher resolution and mass accuracy of the orbitrap analyzer compared to previously employed Q-TOF instrumentation provided high confidence in bacterial proteoform and species identification by top-down protein analysis.
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